Anti-Ageing Efficacy of Human Growth Factors and Cytokines Containing Cosmetic Skin Serum in Indian Females
Introduction
Use of growth factors for preventing the aging of skin is an emerging concept. Stem cells release a host of growth factors into the culture medium. These factors provide a microenvironment that favors tissue repair and regeneration and thus helps in skin rejuvenation and reversal of photageing.
Aim
To evaluate the anti-ageing efficacy and safety of a cosmetic serum containing conditioned medium obtained from adult human bone marrow derived allogenic mesenchymal stromal cells (BMMSC).
Patient Profile
Indian female aged 35 to 60 years, belonging to skin type IV - VI
Methods
- Open label, monocentric, single arm study
- N=40 females
- Serum containing growth factors and cytokines was applied twice daily (morning and evening)
- The study was conducted for a period of approximately 90 days for each subject
- Dermatologist’s assessment for efficacy was done using dermatological assessments questionnaire
- Parameters considered for efficacy assessment were fine lines, wrinkles, crow’s feet, evenness of skin tone, skin firmness/laxity, hydration of skin, homogeneity of age spots, and visible pores
- Instrumental assessment
- Standard non-invasive instruments Chromameter, Cutometer, Corneometer and Visioscan were used to assess skin colour, elasticity, hydration and wrinkles respectively.
- Safety and tolerability assessment
Results
- Twice daily application of serum containing growth factors and cytokines for a period of 90 days considerably improved tested anti-ageing parameters
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Table 1: Efficacy results: Dermatologist scores and Instrumental scores for various parameters
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Dermatologist score |
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Baseline |
Day 15 |
Day 30 |
Day 60 |
Day 90 |
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Fine lines and wrinkles Score (P-value) |
3.24 |
3.26 |
3.58 (p<0.001) |
3.79 (p <0.001) |
4 (p <0.001) |
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Crow’s feet Score (p-value) |
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Right |
1.79 |
1.68 (0.04) |
1.45(p= 0.0003) |
1.47 (p= 0.0007) |
1.32 (p <0.0001) |
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Left |
1.79 |
1.71 (p=0.083) |
1.53(p= 0.0008) |
1.5(p <0.0004) |
1.39 (p <0.0001) |
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Evenness of skin tone Score (P-value) |
2.76 |
2.82 |
3 (p=0.0017) |
3.16 (p<0.0001) |
3.45 (p<0.0001) |
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Skin firmness/Laxity score (P-value) |
2.42 |
2.45 |
2.68 (p= 0.0008) |
2.84 (p <0.0001) |
2.92 (p <0.0001) |
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Hydration of skin score (P-value) |
2.97 |
3.5 (p <0.0001) |
3.92 (p <0.0001) |
3.92 (p <0.0001) |
3.95 (p <0.0001) |
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Homogenecity of age spots score (p-value) |
2.89 |
2.97 (p= 0.0831) |
3.08 (p= 0.0064) |
3.18 (p= 0.0004) |
3.24 (p <0.0001) |
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Instrumental score |
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Corneometer assessment of skin hydration (p-value) |
Cheek =54.43 Forehead=45.67
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- |
Cheek=62.49 Forehead=53.93 (p <0.0001) |
Cheek= 65.58 Forehead= 58.67 (p <0.0001) |
Cheek=67.21 Forehead=64.72 (p <0.0001) |
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Skin evaluation of wrinkles using Visioscan score (P-value) |
36.72 |
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36.75 |
35.69 (p= 0.0027) |
34.77(p <0.0001) |
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Chromameter measurement of luminescence |
Cheek= 49.87 Forehead= 48.23
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Cheek= 50.76 (p<0.0001) Forehead= 48.85 (p<0.0001)
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Cheek= 50.76(p<0.0001) Forehead= 48.76(p<0.0001)
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Cheek= 50.73(p<0.0001) Forehead= 48.56 (p=0.0397)
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Cutometer - skin elasticity |
R2=0.64 R5=0.72 R6=0.83 |
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R2=0.69 R5=0.72 R6=0.71 (p=0.0302) |
R2=0.79 (p<0.0001) R5=0.78 R6=0.54 (p<0.0001) |
R2=0.77 (p<0.0001) R5=0.84 (p<0.0001) R6=0.69 (p=0.0189) |
R2 and R5 values correspond to overall or net elasticity of the skin. The closer the value is to 1.0, higher is the skin elasticity.
R6 value stands for visco-elasticity of the skin. This value should decrease as a sign of improvement.
Figure 3: Proportion of subjects felt improvement in several parameters
Safety
- Site application reaction by dermatological evaluation for product tolerance showed that one subject had mild dryness at baseline and day 30.
- One subject had mild acneiform eruption at day 15 and day 30.
- Frequency and severity of the noted reactions were neither clinically significant nor required any medical intervention.
- At day 60, no site application reaction was noted by the dermatologists or perceived by the subjects and the product was considered overall safe.
Conclusion
- Twice daily application of serum containing growth factors and cytokines for a period of 90 days Indian females was safe and well tolerated
- It can be used as either an alternative or as an adjunct to invasive rejuvenation procedures such as laser resurfacing
J. cosmet. dermatol. sci. appl. 2017, 7, 118-132
